Figure 4 | Cell Death & Disease

Figure 4

From: ID2 promotes survival of glioblastoma cells during metabolic stress by regulating mitochondrial function

Figure 4

ID2 protects mitochondria from damage following GluDep. (a) Representative fluorescent micrographs of mitochondria in LN229(shNC) and LN229(shID2) cells stained by TMRM (red) following GluDep for the times indicated. DNA was counterstained with Hoechst 33258 (blue). Inset scale bar: 200 μm. (b) Mitochondrial damage measured by decreased TMRM fluorescence in LN229(shNC) and LN229(shID2) cells following GluDep for the times indicated. (c) Mitochondrial damage in LN229(shNC) and LN229(shID2) cells following ionomycin treatment for 30 min at the indicated doses. (d) Mitochondrial damage in LN229(shNC) and LN229(shID2) cells following ionomycin treatment (6 μmol/l) for the times indicated. (e) In vitro calcium-induced swelling of mitochondria isolated from LN229(shNC) and LN229(shID2) cells. The swelling assays were performed three times and representative data are shown as mean±S.D. from four replicates in one assay. (f) Relative mitochondrial DNA copy number in LN229(shNC), LN229(shID2), and ρ(0) cells. (g) Viability of LN229(shNC), LN229(shID2), and ρ(0) cells following GluDep for 8 h. *P<0.05; **P<0.01

Back to article page