Figure 5 | Cell Death & Disease

Figure 5

From: ID2 promotes survival of glioblastoma cells during metabolic stress by regulating mitochondrial function

Figure 5

ID2 suppresses mitochondrial oxidative respiration and mitochondrial ATP production. (a–b) Mitochondrial membrane potential in LN229(shNC) and LN229(shID2) cells was determined by flow cytometric measurement of the mean intensity of TMRM (a) or MitoTracker Orange (b) fluorescence. (c) Mitochondrial oxidative respiration in LN229(shNC) and LN229(shID2) cells determined by the Seahorse XF24 analyzer. The chart of oxygen consumption rates (OCR) versus time as shown is representative of four independent experiments in which five replicates of each assay were evaluated. Basal respiration (d), maximal respiration (e), proton leak (f), and mitochondrial ATP production (g) were calculated. (h) Mitochondrial ATP production of LN229(shNC) and LN229(shID2) cells in the presence of DMSO or 1 μmol/l oligomycin was determined by ATP luminescence. **P<0.01; ***P<0.001; n.s., no significance

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