Figure 3

Overexpression of EphB2 in hippocampus ameliorates learning and memory deficits in APP/PS1 transgenic mice. (a and b) The lentivirus was injected into dorsal hippocampus and the neurons were well infected with Lenti-GFP. (c) Quantification of EphB2-Flag expression showed no difference between APP/PS1 transgenic mice and WT mice (n=9–10). (d and e) APP/PS1 mice injected with Lenti-EphB2 spent less time on reaching the platform than that of Lenti-empty after training (n=10 in each group). The latency to first reach the platform was recorded 24 h after last training session. Lenti-empty-treated APP/PS1 transgenic mice spent more time on reaching the platform compared to corresponding WT mice. While Lenti-EphB2-treated APP/PS1 transgenic mice performed less time. (n=10 in each group). (f) Overexpression of EphB2 significantly improved the impaired context-dependent fear memory in APP/PS1 transgenic mice (n=10 in each group). (g) Overexpression of EphB2 significantly improved the impaired tone-dependent fear memory in APP/PS1 transgenic mice (n=10 in each group). *P<0.05, ***P<0.001 versus Lenti-empty-treated WT group; ^#P<0.05, ^###P<0.001 versus Lenti-empty-treated APP group. Data are presented as mean±S.E.M.