Figure 1
From: An enhanced bioluminescence-based Annexin V probe for apoptosis detection in vitro and in vivo
Pathways of apoptosis (extrinsic – blue box and intrinsic – orange box) and detection concept. Both pathways converge to the activation of caspases that are principally responsible for morphological and biochemical changes associated with apoptosis including PS exposure. Although PS is sequestered to the inner-face of the plasma membrane under healthy conditions, the ArFP construct exhibits the ability to bind PS at surface of apoptotic cells and generate a bioluminescent signal in the presence of substrate coelenterazine
