Figure 2
From: An enhanced bioluminescence-based Annexin V probe for apoptosis detection in vitro and in vivo
(a) SDS-PAGE analysis of components and fusion protein (lane 1: Annexin V, lane 2: RLuc8, lane 3: ArFP, lane 4: Precision Plus Protein Ladder (Bio-Rad)). (b) Western blot analysis of components and fusion protein identified using anti-Annexin V antibody (lane 1: Precision Plus Protein Ladder, lane 2: Annexin V, lane 3: RLuc8, lane 4: ArFPP) or anti-Renilla luciferase antibody (lane 5: Precision Plus Protein Ladder, lane 6: Annexin V, lane 7: RLuc8, lane 8: ArFP). (c) Far-UV CD spectra of ArFP (purple), Annexin V (teal), and RLuc8 (blue). (d) Protein 2° structure assignment (CDSSTR analysis via Dichroweb) for ArFP. (e) Thermostability analysis (ellipticity at 222 nm) of ArFP. (f) Comparison of Annexin V and ArFP thermostability (upper) and first derivative analysis of curves (lower). (g) Comparison of RLuc8 and ArFP thermostability (upper) and first derivative analysis of curves (lower)
