Figure 3
From: An enhanced bioluminescence-based Annexin V probe for apoptosis detection in vitro and in vivo
(a) Top: ITC data from the titration of 30 μM ArFP with lipid vesicles composed of 60:40 mixture of POPC:POPS in the presence of 0.75 mM Ca2+ at 18 °C. Middle: Heat of dilution (control/baseline). Bottom: Integrated heats of binding as a function of ligand:protein mole ratio. (b) Bioluminescence spectra from 400 to 700 nm resulting from addition of coelenterazine substrate to ArFP (purple) or RLuc8 (blue). (c) Kinetic bioluminescence data resulting from injection of coelenterazine substrate to ArFP (purple) or RLuc8 (blue) (250 integrations at 0.24 s/each). (d) Concentration-dependent bioluminescence output comparison of ArFP (purple) or RLuc8 (blue) after injection of coelenterazine substrate (10 s integration). All points are the mean of three measurements±one S.D. Error bars that are not visible are obstructed by the data point
