Figure 4 | Cell Death & Disease

Figure 4

From: Long noncoding RNA UCA1 induced by SP1 promotes cell proliferation via recruiting EZH2 and activating AKT pathway in gastric cancer

Figure 4

Cyclin D1 mediated the regulation of UCA1 on G1/S transition. (a) NCI-N87 cells co-transfected pcDNA-UCA1 and cyclin siRNAs showed significantly decreased ratio of cell in S phase than NCI-N87 cell with pcDNA-UCA1 alone and accumulation of cells in G0/G1 phase (**P<0.01). (b) EdU dye assay was performed to further determine the inhibition of G1/S transition in NCI-N87 cells co-transfecetd with pcDNA-UCA1 and cyclin D1 siRNAs. Cylin D1 siRNAs significantly reverse the regulation of UCA1 on G1/S transition in NCI-N87 (**P<0.01). (c and d) Western blot and qRT-PCR were used to determine the effect of cyclin D1 on NCI-N87 cell transfected with pcDNA-UCA1. Cylin D1 siRNAs inhibited the expression of cyclin D1 both in mRNA and protein level to partly reverse the modulation of UCA1 on cyclin D1 in NCI-N87 cell (**P<0.01). Error bars represent the mean±S.D. of triplicate experiments

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