Figure 6 | Cell Death & Disease

Figure 6

From: Long noncoding RNA UCA1 induced by SP1 promotes cell proliferation via recruiting EZH2 and activating AKT pathway in gastric cancer

Figure 6

UCA1 interacts with EZH2 in GC. (a) Distribution of UCA1 in GC was determined by qRT-PCR after isolating nucleus RNA and cytoplasm RNA. UCA1 are both localized in nucleus and cytoplasm. (b) RNA pull-down assay was performed to determine the association between UCA1 and EZH2. UCA1 RNA-bound EZH2 protein was detected via western blot and no band showed up in negative control (**P<0.01). β-actin acted as the loading control. (c) RIP assay was used to further confirm the interaction between UCA1 and EZH2. Anti-EZH2 significantly harbored more UCA1 fragments than anti-IgG via RT-PCR (**P<0.01). Error bars represent the mean±S.D. of triplicate experiments. Anti-SNRNP70 was used as a positive control

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