Figure 2
cPLA2α overexpression enhanced breast cancer cells migration and invasion in vitro. (a) Assessment of the transfected efficiency of cPLA2α protein expression after retroviral infection in T47D cells. (b) Scratch assay comparing the migration of SCR/T47D (left) and overcPLA2α/T47D (right) cells. (c) Comparison of chemotaxis potential of SCR/T47D (left) and overcPLA2α/T47D (right) cells under the application of high concentration of FBS (20%) with constant stimulation for 6 h. (d) Comparison of the invasion potential of SCR/T47D (left) and overcPLA2α/T47D (right) cells after incubation with EGF (10 ng/ml) at 37 °C in 5% CO2 for 36 h by counting the number of the cells that invaded through Matrigel-coated transwell inserts. **P<0.01, ***P<0.001. Scale bar, 1.0 mm. All experiments were repeated at least three times
