Figure 3
cPLA2α regulated MDA-MB-231 cell migration and invasion capacities in vitro. (a) Assessment of the transfected efficiency of cPLA2α protein expression after retroviral infection in MDA-MB-231 cells. (b) Repression specificity of cPLA2α mRNA expression in MDA-MB-231 cells by qRT-PCR. (c) Scratch assay comparing the migration of SCR/MDA-MB-231, sicPLA2α /MDA-MB-231 and overcPLA2α/MDA-MB-231 cells. (d) Comparison of chemotaxis potential of SCR/MDA-MB-231, sicPLA2α MDA-MB-231 and overcPLA2α/MDA-MB-231 cells under the application of high concentration of FBS (20%) with constant stimulation for 4 h. (e) Comparison of the invasion potential of SCR/MDA-MB-231, sicPLA2α/MDA-MB-231 and overcPLA2α/MDA-MB-231 cells after incubation with EGF (10 ng/ml) at 37 °C in 5% CO2 for 24 h by counting the number of the cells that invaded through Matrigel-coated transwell inserts. (f) Comparison of the proliferation of SCR/MDA-MB-231, sicPLA2α/MDA-MB-231 and overcPLA2α/MDA-MB-231 cells by Cell Counting Kit-8 (CCK8) assay. **P<0.01 and ***P<0.001. Scale bar, 1.0 mm. All experiments were repeated at least three times
