Figure 4 | Cell Death & Disease

Figure 4

From: WDR79 promotes the proliferation of non-small cell lung cancer cells via USP7-mediated regulation of the Mdm2-p53 pathway

Figure 4

WDR79 stabilizes Mdm2 and p53 by protecting them from proteasome-mediated degradation. (a and b) A549 cells transfected with the indicated constructs (a) or infected with lentivirus encoding the indicated shRNA (b) were treated with DMSO or MG132 for 6 h. Expression of the indicated protein was examined by western blotting using the indicated antibodies. (c and d) H1299 cells transfected with the indicated constructs (c) or infected with lentivirus encoding the indicated shRNA (d) were treated with DMSO or MG132 for 6 h. Expression of the indicated protein was examined by western blotting using the indicated antibodies. (e and f) A549 (e) and H1299 (f) cells infected with lentivirus encoding the indicated shRNA were treated with cycloheximide (50 μg/ml), harvested at the indicated point and then immunoblotted with the indicated antibodies. (g and h) Quantification of the p53 or Mdm2 protein levels relative to GAPDH. Intensities of blots were analysed by the ImageJ software

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