Figure 6

WDR79 mediates Mdm2 and p53 stabilization in a UPS7-dependent manner. (a and b) A549 (a) or H1299 (b) cells infected with lentivirus encoding the indicated shRNA were transfected with the indicated constructs. Lysates were blotted with the indicated antibodies. (c and d). A549 (c) or H1299 (d) cells infected with the lentivirus encoding the indicated shRNA were treated with MG132 for 6 h. Immunoprecipitation were performed with anti-USP7 antibody. The immunoprecipitates were then blotted with the indicated antibodies. (e and f) A549 (e) or H1299 (f) cells infected with lentivirus encoding the indicated shRNA were transfected with the indicated constructs. Lysates were blotted with the indicated antibodies