Figure 5
From: Knockout of Eva1a leads to rapid development of heart failure by impairing autophagy
Assessment of autophagy and apoptosis in Eva1a-deficient mice. (a) Representative western blot analysis of Lc3b and Sqstm1 in heart extracts obtained from different groups. (b and c) Densitometric analysis of Sqstm1 and Lc3b-II (*P<0.05, **P<0.01, n=4). (d) Representative images of TUNEL staining (green) and Hoechst staining (blue) of nuclei in cryosectioned heart tissue. (e) Quantification of positive cells displaying terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling (TUNEL) staining. (f) Ultrastructural images reveal the presence of swollen mitochondria and lipid accumulation in Eva1a-deficient mice. (g) Detection of ATP levels (*P<0.05, n=3)
