Figure 1 | Cell Death & Disease

Figure 1

From: Foot-and-mouth disease virus infection inhibits LGP2 protein expression to exaggerate inflammatory response and promote viral replication

Figure 1

FMDV infection triggers LGP2 mRNA and decreases LGP2 protein expression levels. (a, b) PK-15 cells were mock-infected or infected with FMDV (0.5 MOI) for 0, 2, 4, 8, 12 or 24 h, and LGP2 mRNA and viral RNA were detected by qPCR. (c) PK-15 cells were infected with FMDV (0.5 MOI) for 0, 2, 4, 8, 12 or 24 h, and endogenous LGP2 and viral VP1 proteins were detected by western blotting. (d) PK-15 cells were transfected with Myc-vector (2 μg) or Myc-LGP2 plasmids (2 μg), and Myc-LGP2-transfected cells were mock-infected or infected with 0.05 or 0.5 MOI of FMDV at 12 hpt. Expression of Myc-LGP2 and viral VP1 proteins was detected by western blotting at 12 hpi. The change in abundance of Myc-LGP2 in the transfectants was analysed by densitometric analysis using ImageJ Software and normalized to β-actin (right panel).

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