Figure 2 | Cell Death & Disease

Figure 2

From: Foot-and-mouth disease virus infection inhibits LGP2 protein expression to exaggerate inflammatory response and promote viral replication

Figure 2

Overexpression of LGP2 suppresses FMDV replication. (a) PK-15 cells were transfected with Myc-vector (2 μg) or Myc-LGP2 plasmids (2 μg), and the transfected cells were infected with FMDV (0.5 MOI) at 24 hpt. Expression of Myc-LGP2 and viral VP1 proteins was detected by western blotting, and viral RNA was detected by qPCR, at 12 hpi. (b) PK-15 cells were transfected with increasing amounts of Myc-LGP2 plasmids (0, 1, 2 or 3 μg) and the Myc-vector was used in the transfection process to ensure that the cells received the same amounts of total DNA plasmids. The transfected cells were infected with FMDV (0.5 MOI) at 24 hpt. Expression of Myc-LGP2 and viral VP1 proteins was detected by western blotting, and viral RNA was detected by qPCR, at 12 hpi; (c) and the viral titres were detected by TCID50 assay. All the experiments were repeated three times with similar results.

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