Figure 4 | Cell Death & Disease

Figure 4

From: Foot-and-mouth disease virus infection inhibits LGP2 protein expression to exaggerate inflammatory response and promote viral replication

Figure 4

Loss of LGP2 promotes inflammatory response and FMDV replication. (a) PK-15 cells were transfected with NC siRNA or LGP2 siRNA, and expression of endogenous LGP2 protein was detected at 0, 36 and 48 hpt. (b) PK-15 cells were transfected with NC siRNA or LGP2 siRNA for 36 h, followed by infection with equal amounts of FMDV (0.5 MOI) for 0, 10 and 18 h. Expression of endogenous LGP2 and viral proteins was detected by western blotting. Expression of LGP2 mRNA and viral RNA was detected by qPCR. (c) Expression of CCL3L1 and TNF-α mRNA in the NC siRNA- and LGP2 siRNA-transfected cells infected by FMDV for 18 h was detected by qPCR. (d) Alignment of the LGP2 genomic reference sequence, the LGP2-WT, LGP2-KO-1 and LGP2-KO-2 DNA sequences using LaserGene software. The red box indicates the regions that were mutated. (e) Confirmation of successful knockout of LGP2 in the LGP2-KO cell line by western blotting. (f) Equal amounts of LGP2-WT and LGP2-KO cells were infected by FMDV (0.5 MOI) for 10 h, and viral protein (left panel) and RNA (right panel) were detected. (g) Expression of CCL3L1 and TNF-α mRNA were determined.

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