Figure 6 | Cell Death & Disease

Figure 6

From: Foot-and-mouth disease virus infection inhibits LGP2 protein expression to exaggerate inflammatory response and promote viral replication

Figure 6

Functional domain of LGP2 to suppress FMDV replication. (a) Schematic representation of a series of Myc-tagged LGP2 mutant constructs. (b) PK-15 cells were transfected with the empty vector (2 μg), Myc-LGP2 (2 μg) or various LGP2 mutant plasmids (2 μg) for 24 h, and followed by infection with FMDV (0.5 MOI) for 12 h. Viral RNA levels were detected by qPCR. (c) HEK293T cells were co-transfected with Myc-LGP2 plasmids (2 μg) or various LGP2 mutant plasmids (2 μg) and Flag-2B plasmid for 36 h. Expression of Myc-LGP2, Myc-tagged LGP2 mutants and Flag-2B proteins was detected by western blotting.

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