Figure 7 | Cell Death & Disease

Figure 7

From: Foot-and-mouth disease virus infection inhibits LGP2 protein expression to exaggerate inflammatory response and promote viral replication

Figure 7

Functional region of 2B responsible for inducing reduction of LGP2. (a) Schematics of a series of Flag-tagged 2B mutant constructs. (b) HEK293T cells were co-transfected with Myc-LGP2 plasmid (2 μg) and Flag-2B or Flag-tagged 2B mutant plasmids (2 μg) for 36 h. Expression of Myc-LGP2, Flag-2B and Flag-tagged 2B mutant proteins were detected by western blotting. (c) PK-15 cells were transfected with the plasmids expressing Flag-2B or truncated mutants, and the cells were infected with equal amounts of FMDV at 12 hpt. Expression of CCL3L1 and TNF-α was detected by qPCR at 18 hpi.

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