Figure 3

Expression of metabolism-relevant transcriptional targets of PPARα is restored upon dual nuclear receptor activation. C57BL/6 WT and miR-21 KO mice were fed a FF diet (n=12) or a control diet (SD; n=12), with or without supplementation with OCA (10 mg/kg/day) for 25 weeks. (a) qRT-PCR analysis of Cyp4a14, FAT, ACOX-2 and CPT-1. (b) ROS levels (top) and qRT-PCR analysis of HO-1 (bottom). Results are expressed as mean±S.E.M. fold change. *P<0.05 and ***P<0.001 from WT control mice; ^†P<0.05, ^‡P<0.01 and ^ϕP<0.001 compared with WT FF-fed mice. SD, standard diet