Figure 4

Hepatic insulin sensitivity is restored upon PPARα and FXR activation. C57BL/6 WT and miR-21 KO mice were fed a FF diet (n=12) or a control diet (SD; n=12), with or without supplementation with OCA (10 mg/kg/day) for 25 weeks. (a) Immunoblotting of p-JNK. Representative blots are shown. Blots were normalized to total JNK. (b) Immunoblotting of p-IRS1 (left) and p-INSR (right). Representative blots are shown. Blots were normalized to total IRS1 and INSR respectively. (c) Immunblotting of p-AKT. Representative blots are shown. Blots were normalized to total AKT. Results are expressed as mean±S.E.M. fold change. *P<0.05 and **P<0.01 from WT control mice; ^†P<0.05 and ^ϕP<0.001 compared with WT FF-fed mice. SD, standard diet