Figure 6
From: miR-21 ablation and obeticholic acid ameliorate nonalcoholic steatohepatitis in mice
miR-21 expression is increased in the liver, muscle and serum of NAFLD patients. (a) qRT-PCR analysis of liver miR-21. (b) Representative PPARα immunostaining (red) in liver tissue from patients. Nuclei were counterstained with Hoechst 33258 (blue). The corresponding histogram shows the quantification of PPARα mean fluorescence intensity, as described in Materials and Methods section. At least five images per liver sample were used. Scale bar=10 μm. (c) qRT-PCR analysis of muscle miR-21. (d) qRT-PCR analysis of serum miR-21. Results are expressed as mean±S.E.M. fold change. *P<0.05 and **P<0.01 from steatosis
