Figure 5

Growth inhibition of intracranial tumor by CTS treatment extended survival of nude mice bearing intracerebral U87 xenografts treated with CTS. (a) Growth inhibition of intracranial tumor by CTS treatment. Left: after hematoxylin–eosin staining, tumor size was determined by the areas that were measured at the maximal brain tumor dimensions in the coronal sections. The red circles indicate tumor tissue. Scale bar, 1 mm. Data were calculated by taking the tumor size of control as 100%. Data are expressed as mean±S.E.M., n=5 for each group. *P<0.05; **P<0.01 versus Model control group. Right: higher magnification in HE staining. Scale bar, 50 μm. (b) After xenografts for 7 days, nude mice were treated with CTS from day 8 to 21 and were observed after discontinuation of therapy. Statistical significance was achieved by Cox–Mantel and Wilcoxon analyses of a Kaplan–Meier survival curve (n=5). (c) Some samples from the harvested tumor tissue on Day 28 were used in detecting tyrosine phosphatase activity of SHP-2. Data are expressed as mean±S.E.M., n=3 for each group. *P<0.05; **P<0.01, versus Model control group