Figure 2

miR-218-5p promotes sensitivity of gemcitabine in NOZ and GBC-SD cell lines. (a) MTS analysis of GBC cells (NOZ and GBC-SD) transiently transfected with miR-218-5p mimic (Mimic-miR-218-5p), negative control mimic (Mimic-Con), miR-218-5p antisense (As-miR-218-5p), or negative control antisense (As-Con). (b–c) Representative images of colony formation and the mean fold change of colonies induced by miR-218-5p mimic or antagomir in GBC cells. (d–g) MTS assay and calculating the corresponding IC50 of gemcitabine in GBC cells transfected with miR-218-5p mimic or antagomir. (h–k) Flow cytometric analysis of Annexin V/PI staining used to quantify apoptosis rate of GBC cells transfected with miR-218-5p mimic or antagomir, followed by exposure to gemcitabine (NOZ, 10 μM; GBC-SD, 1 μM) for 72 h. All n=3; bar, S.E.M., NS, not significant, *P<0.05; **P<0.01; ***P<0.001; Student’s t-test