Figure 4

miR-218-5p-sensitized GBC cells to gemcitabine was mediated by PRKCE. (a–d) Efficiency testing of overexpression or interference of PRKCE in GBC cells by using Q-PCR and western blot. (e-h) MTS assay and calculating the corresponding IC50 of gemcitabine in GBC cells transfected with PRKCE construct or PRKCE siRNAs. (i, j) Flow cytometric analysis of Annexin V/PI staining used to quantify apoptosis rate of GBC cells transfected with PRKCE construct or PRKCE siRNAs, followed by exposure to gemcitabine (NOZ, 10 μM; GBC-SD, 1 μM) for 72 h. (k, l) IC50 of gemcitabine in GBC cells co-transfected with miR-218-5p mimic and PRKCE construct. GAPDH was used to normalize the Q-PCR results, and β-actin was the loading control in western blot assay. All n=3; bar, S.E.M. *P<0.05; **P<0.01; ***P<0.001; Student’s t-test