Figure 5

miR-218-5p negatively regulates resistance to gemcitabine through PRKCE/MDR1 axis in GBC cells. (a–d) Q-PCR and western blot to analyze MDR1, MRP1, and BCRP mRNA and protein expression, respectively, in GBC cell lines when transfected with miR-218-5p mimic or antagomir. (e, f) Q-PCR and western blot to analyse MDR1 mRNA and P-gp expression, respectively, in GBC cell lines when transfected with PRKCE construct or PRKCE siRNAs. (g–i) Q-PCR and western blot to detect MDR1 mRNA and P-gp expression, respectively, in GBC cell lines when co-transfected with miR-218-5p mimic and PRKCE construct. (j) IC50 of gemcitabine in GBC cells when co-transfected with miR-218-5p mimic and MDR1 construct. GAPDH was used to normalize the Q-PCR results, and β-actin was the loading control in western blot assay. All n=3; bar, S.E.M. *P<0.05; **P<0.01; Student’s t-test