Figure 1
Hydrogen sulfide promotes autophagy of HepG2 and HLE cells. (a) Western blotting detected increased expression of LC3-II, Atg5 and P62 autophagy-related protein in the presence of 10−3 M NaHS in HepG2 and HLE cells. β-actin was used as internal control. (b) Densitometric analysis of LC3-II/LC3-I is shown in the histogram. (c) HepG2 and HLE cells transfected with GFP-LC3 plasmid after 24 h, LC3 puncta dots (green) were observed under a fluorescence microscope; the nuclei (blue) were stained with DAPI. (Scale bar: 20 μm) (d) The percentage of cells presenting typical LC3 puncta dots. (e) Expression of P62 as analyzed by fluorescence microscopy in HepG2 and HLE cells treated with NaHS for 24 h (Scale bar: 100 μm). (f) Intracellular double-membrane vesicles (arrows), the ultrastructural feature of autophagy, were observed by TEM (Scale bars 2 and 1 μm). Each figure is representative of an experiment that was repeated at least three times. The data represent the mean±S.D. of three samples. *P<0.05 compared with control
