Figure 3
H2S promotes cell autophagy by inhibiting the PI3K/AKT/mTOR signaling pathway. (a) Western blotting was used to detect the expression of PI3K/ p-PI3K, Akt/p-Akt and mTOR in HepG2 and HLE cells. (b) Immunofluorescence staining revealed that p-PI3K/p-Akt/mTOR decreased after NaHS treatment, with the green indicating positive staining for p-PI3K/p-Akt/mTOR (Scale bar: 40 μm). (c) Western blotting was performed to detect the expression of LC3-II in HepG2 and HLE cells treated with NaHS alone, rapamycin alone or NaHS in combination with rapamycin. β-actin was used as internal control. (d) Densitometric analysis of LC3-II was shown as a histogram. The data represent the mean±S.D. of three samples.*P<0.05 compared with control cells; #P<0.05 versus cells treated with NaHS alone; &P<0.05 versus cells treated with rapamycin alone. (e) Transmission electron microscope observation of cell autophagosomes (Scale bar: 2 μm)
