Figure 6
Characterization of photo-activation on D112-induced apoptosis. (a) Quantitation of cell death morphologies in SK-BR-3 cells. Cells were treated with 1 μg/ml D112 for 1 h, washed, photo-activated for the indicated amounts of time (0-60 s) and cell death morphologies were quantitated at indicated times. (b) Kinetics of apoptosis-induction by photo-activated D112 in SK-BR-3 cells. Cells were treated with 1 μg/ml D112 for 1 h, photo-activated for 10 s, and percent apoptotic cells were recorded for the indicated time points. (c) Dose-dependent analysis of photo-activated D112-induced apoptosis in SK-BR-3 and MDA-MB-468 cells. Cell lines were treated with D112 at the indicated concentrations for 1 h, washed, photo-activated for 10 s, and percent apoptotic cells were quantified at the indicated timepoint. Each data point is an average of three random fields of view per experiment taken from three independent experiments. All values are mean±S.D. of three independent experiments
