Figure 4 | Cell Death & Disease

Figure 4

From: Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway

Figure 4

Activation of CaSR blocks HG-induced Cx43 degradation and stabilizes function of cell gap junction. HG-induced degradation of β-catenin, N-cadherin and Cx43. NPS R568 could decrease the effect of HG. (a) Representative western blot of β-catenin, N-cadherin and Cx43 in comparison with Na+, K+-ATPase expression in cardiomyocytes exposed to HG in the presence of 5 μM NPS R568 or 3 μM Calhex231 (n=3). (b) The expression of β-catenin and N-cadherin complex were detected (n=3). A typical blot is shown. (c) The function of Cx43 was measured by scrape-loading dye transfer technique (SLDT) and photoed by fluorescence microscopy. Scale bars=10 μm (n=3). The yellow arrow represents the scratch. (d) The extracellular ATP content of each group was detected by chemiluminescence (n=3). *P<0.05 versus control; #P<0.05 versus HG

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