Figure 5 | Cell Death & Disease

Figure 5

From: Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway

Figure 5

Activation of CaSR inhibits the phosphorylation of GSK-3β and the nuclear translocation of β-catenin induced by HG. HG promoted the nuclear translocation of β-catenin, which was attenuated by NPS R568 pretreatment. (a) The whole-cell proteins from primary neonatal rat cardiomyocytes in control group (5.5 mM) and HG group (40 mM) after various treatment conditions for 48 h were detected by immunoblotting with antibodies against N-β-catenin, Cyto-β-catenin, p-β-catenin, p-GSK-3β and total GSK-3β. A representative blot was shown. (b) The protein levels of N-β-catenin were quantified as a ratio against Histone H3. The expression of Cyto-β-catenin and p-β-catenin were quantified as a ratio against β-actin. The expression of p-GSK-3β were quantified as a ratio against total GSK-3β (n=3). *P<0.05 versus control; #P<0.05 versus HG

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