Figure 6 | Cell Death & Disease

Figure 6

From: Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway

Figure 6

Activation of CaSR can attenuate the degradation of Mfn1, Mfn2 and Cx43 by HG-gp78-ubiquitin proteasome pathway. The expression of gp78 and the ubiquitination level of Mfn1, Mfn2, Cx43 were detected in cultured cardiomyocytes in control group (5.5 mM) and HG group (40 mM) after various treatment for 48 h. (a) Representative western blot of gp78 in comparison with β-actin expression in cardiomyocytes exposed to HG in the presence of 5 μM NPS R568 or 3 μM Calhex231 (n=3); (b) Representative western blot of gp78 in cardiomyocytes which were transfected with gp78-siRNA or Con-siRNA (n=3). (c) The ubiquitination level of Mfn1, Mfn2 and Cx43 in cardiomyocytes which were transfected with gp78-siRNA or Con-siRNA and treated by 5 μM NPS R568 or 3 μM Calhex231 respectively by immunoprecipitation (n=3). A representative blot is shown. *P<0.05 versus control; #P<0.05 versus HG

Back to article page