Figure 5
5-day MSC sheets enhance bone callus formation and improve the biomechanical torsional properties of grafted femurs at 6-weeks post-surgery. (a) The left panels are representative x-ray images of allografts alone, allografts wrapped with lgG cultured 5-day sheets (Day5 sheets), and allografts wrapped with Notch activation cultured 5-day MSC sheets (Day5 sheet/JAG1) at 6-weeks post-surgery. Right panels show the histological sections stained with AB/H/OG. Scale bars=400 μm. Bone callus is marked with black triangles and cartilaginous callus is marked with black arrows. (b) Representative Micro-CT volumetric rendering of the grafted femurs with 5-day lgG or JAG1 MSC sheets at 6-weeks post-surgery. Allograft alone was used as the control. (c) Quantification of bone volume/total tissue volume (BV/TV) from the mineralized calluses of each group. (*, P<0.05 compared with allograft alone; #, P<0.05 compared with allograft+Day5 sheets). (d) Maximal torque and (e) Torsional rigidity of grafted femurs from each group was retrieved upon mice (n=6) sacrifice at 6-weeks post-surgery. Data presented as mean±standard deviation. *P<0.05 compared with allograft alone; #P<0.05 compared with allograft+Day5 sheets
