Figure 3
From: Cytoplasmic RAP1 mediates cisplatin resistance of non-small cell lung cancer
RAP1 acts through NF-κB signaling to support NSCLC cell growth. (a and b) Western blotting analysis (a) and IF assay (b) of NF-κB signaling factors: p65, phosphorylated-p65 (pp65), IκBα, and phosphorylated-IκBα (p-IκBα) in the cytoplasmic and nuclear fractions of A549 cells transduced with shRAP1 or scramble shRNA. Scale bar: 5 μm. (c) Expression of genes positively regulated by NF-κB signaling in A549 cells transduced with shRAP1 or scramble shRNA. Statistics were generated from three independent experiments. **P<0.01, ***P<0.001, Student’s t-test; error bar: ±S.D.
