Figure 4 | Cell Death & Disease

Figure 4

From: Protein disulfide isomerase-mediated apoptosis and proliferation of vascular smooth muscle cells induced by mechanical stress and advanced glycosylation end products result in diabetic mouse vein graft atherosclerosis

Figure 4

The effects of PDI overexpression or PDI inhibitor on changed ox-PDI and total PDI in VSMCs induced by SS and/or AGEs. (a–f) VSMCs lines with stable PDI overexpression were identified by western blot and then used for experiments of SS and/or AGEs-induced ox-PDI or total PDI analysis; (g–j) quiescent cultured VSMCs pretreated with PDI inhibitor (bacitracin, 0.5 mM) for 1 h were treated with SS, AGEs or both for 10 min, and harvested for ox-PDI analysis or for 1 h, cultured additional 24 h, and then harvested for total PDI analysis by western blot. Beta-actin was set as an internal control; (b, d, f, h and j) statistical results of ratios of ox-PDI/ or total PDI/beta-actin from (a, c, e, g and i) from three independent experiments, respectively. a, P<0.05 versus negative control (NC); b, P<0.05 versus AGEs or SS of the same group; c, P<0.05 versus AGEs or SS of the different group by ANOVA with LSD test. Data are shown as means±SEM

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