Figure 7
From: Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme
Cycloheximide inhibits autophagy and UPR signaling, whereas activating downstream mTOR substrate phosphorylation. (a) Brief treatment with cycloheximide led to increased p62 accumulation at 20 h of NIM811 treatment (quantitation with the actin loading control on the right). (b) Two-hour cycloheximide pretreatment decreased LC3-I and II levels even after prolonged NIM811 treatment (44 h), (c) 10 μM NIM811 stimulated phosphorylation of P70S6K at 2 h and p-EIF2a at 4 h, while 2 h of cycloheximide increased the P-P70S6K level but decreased eIF2a phosphorylation. (d) Two-hour cycloheximide pre-incubation boosted the p-p70S6K level after 24 h, whereas, with rapamycin pretreatment, P70S6K phosphorylation decreased and p62 was degraded more efficiently
