Figure 8
From: Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme
Cycloheximide pretreatment exacerbates cell death induced by prolonged NIM811 treatment. (a) Cycloheximide pretreatment did not improve U251 live cell numbers after 24 h of 10 μM NIM811 incubation. (b) Colony number counts on day 13 indicates that pretreatment with rapamycin significantly increased colony numbers over NIM811 alone treatment (from 0.5 to 5 μM), *P<0.05, whereas, cycloheximide pretreatment substantially decreased colony numbers by 0.5 μM NIM811 incubation, *P<0.05. (c) Microscopic images were obtained on day 13 to demonstrating the morphology of the colony cells. Rapamycin also blocked the vacuolization triggered by NIM811. Scale bar=50 μm
