Figure 8 | Cell Death & Disease

Figure 8

From: DT-13 synergistically enhanced vinorelbine-mediated mitotic arrest through inhibition of FOXM1-BICD2 axis in non-small-cell lung cancer cells

Figure 8

DT-13 and NVB cooperated to suppress tumor growth in NCI-H460 xenograft nude mice. Statistical analysis of relative tumor volume (a), tumor volume (b), nude mice weight (c) and tumor weight (d) was performed, and 10 mg/kg NVB was used as a positive control. (e) Image of resected xenograft tumor after combination treatment was shown. (f) Relative mRNA levels of FOXM1 and BICD2 from tumor tissue were determined by RT-qPCR analysis. (g) The protein levels of PARP, MPM2, FOXM1 and BICD2 were examined by western blotting, and β-actin was served as the loading control. (h) Tumor tissues from NCI-H460-xenografted nude mice after DT-13/NVB treatment were performed by TUNEL assay. Molecular alterations of MPM2, FOXM1 and BICD2 in each group were detected by immunohistochemistry analysis. (i) The quantity of tunnel-positive cells in each visual field was statistically analyzed in the histograms. The data were expressed as mean±S.D. using Student’s t-test (two-tailed). *P<0.05, **P<0.01 and ***P<0.001. RT-qPCR, real-time quantitative PCR

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