Figure 4

Characterization of cultured SSCs from Zmym3 KO mice. (a) Bright-field images of cultured WT and KO SSCs. (b) Western blot of ZMYM3 protein in WT and KO SSCs. (c) Quantitative evaluation of proliferations of WT and KO SSCs. Equal numbers of WT and KO SSCs were seeded on MEF feeder. Cell numbers are counted 5 days later and normalized by the number of WT SSCs. (d) Immunostaining of SYCP3⁺ cells induced from WT and KO SSCs. Germ cell marker MVH was co-immunostained. S-cells (arrows); W-cells (arrow heads). (e) Quantitative comparisons in the percentages of S-cells and W-cells among all induced SYCP3⁺ cells between WT and KO SSCs. Equal numbers of WT and KO SSCs were planted on Sertoli cells. The images were taken on day 6 of induction represented by (d). The ratios of S- and W-cells were calculated based on the results from three independent assays (n=3)