Figure 7
RNA-Seq analysis of the effect of Zmym3 KO on gene expression in cultured SSCs and isolated spermatocytes. (a) c-KIT+ cells were induced from both WT and KO SSCs by 100 nM RA 36 h after RA treatment. Only result for WT SSCs is shown. (b) Gene sets up- or down-regulated by RA in WT and KO SSCs. (c) Gene sets up- or down-regulated by Zmym3 KO in SSCs and induced c-KIT+ cells. (d) Immunostaining of SYPC3 and γH2AX on spermatocytes isolated from WT or KO mice to show that higher than 80% of the cells were double positively stained. Only the result for WT mice is shown. (e) Gene sets up- or down-regulated by Zmym3 in isolated spermatocytes from 5- and 7-month mice. (f–g) qRT-PCR evaluation of the expression of genes identified by RNA-seq analysis
