Figure 2 | Cell Death & Disease

Figure 2

From: Inflammatory mediator ultra-low-molecular-weight hyaluronan triggers necrosis of B-precursor leukemia cells with high surface CD44 expression

Figure 2

Analysis of CD44 splice variants. (a) RT-PCR analysis of CD44 splice variants. mRNAs were extracted from MLL+ ALL (n=7), other B-precursor ALL (n=6), EBV-transformed LCL (n=1) and T-ALL cell lines (n=3), and RT-PCR were performed using primers described in Materials and methods section. The colorectal carcinoma cell line HCT116 was used for positive control. (b) Flow cytometric analysis of CD44v9 expression. Three CD44v mRNA-positive cell lines (KOPB26, KOCL69 and JUTKAT) and one CD44v mRNA-negative cell line (KOPN1) were stained with normal rat IgG (open cytograms) or rat anti-human CD44v9 antibody (filled cytograms) and labeled with FITC-conjugated second antibody

Back to article page