Figure 2 | Cell Death & Disease

Figure 2

From: The putative tumor suppressor microRNA-30a-5p modulates clear cell renal cell carcinoma aggressiveness through repression of ZEB2

Figure 2

ZEB2 is the target of miR-30a-5p. (a) Schematic illustration of the predicted miR-30a-5p binding sites in the 3′-UTR of ZEB2. (b) ZEB2 was the target of miR-30a-5p. MiR report constructs containing one wild-type and two mutated ZEB2 3′-UTRs were co-transfected into 769-P cells, which were infected with either a miR-control-lentivirus or miR-30a-5p lentivirus. Relative repression of firefly luciferase expression was normalized to a transfection control. Results are presented as mean±S.D. and are representative of three independent experiments. (c) ZEB2 mRNA levels after transfection of miR-30a-5p into 769-P cells as measured by real-time PCR. (d) One hundred and twenty ccRCC patients from the First Affiliated Hospital and Cancer Center of Sun Yat-sen University, and (e) five hundred and fifteen ccRCC patients from the TCGA database. ZEB2 expression had an inverse correlation with miR-30a-5p expression in ccRCC patients

Back to article page