Figure 4
Exogenetic expression of miR-30a-5p reduces ccRCC cell line proliferation and metastasis in vivo. (a) Actual sizes of representative tumors. (b) Mean tumor volume (±S.D.) (n=6, *P<0.05 and **P<0.01 comparing exogenetic expression of miR-30a-5p and other treatments). Tumor diameter was measured using a digital caliper and tumor volume was calculated twice a week using the formula volume=W2 × L/2. (c) Mean tumor weight (±S.D.) (n=6, P<0.01 comparing exogenetic expression of miR-30a-5p and other treatments). (d) The number of nodules were quantified in the lungs of athymic mice 6 weeks after tail vein injection of wild-type, miR-control lentivirus-infected and miR-30a-5p lentivirus-infected 769-P cells (n=6 per group). The nodules were examined under an anatomical microscope (P<0.01, Mann–Whitney U-test) (e) Left panel: representative metastatic nodules on the surface of the lungs of athymic mice. Right panel: H&E staining of a section of metastatic tumors in the lung (M) and normal lung (N). Original magnification × 100
