Figure 3
The accumulation of GATA6 is required for DHA to induce HSC senescence in vitro. (a) Activated HSCs were treated with DHA at various concentrations for 24 h or DHA at 20 μM for various hours. Then, western blot was used to determine the expression of GATA6. Activated HSCs were stably transfected with GATA6 siRNA or GATA6 plasmid, and then were treated with 20 μM DHA for 24 h. (b) Western blot was used to determine the expression of GATA6. (c, f) Western blot was used to determine the expression of Hmga1, p53, p21 and p16. (d, g) Real-time PCR was used to show the mRNA expression of Hmga1, p53, p21 and p16. (e, h) β-galactosidase staining was used to detect senescence. For the statistics of each panel in this figure, data are expressed as mean±S.D. (n=3); *P<0.05 versus control, **P<0.01 versus control, ***P<0.001 versus control. #P<0.05 versus DHA treatment, ##P<0.01 versus DHA treatment, ###P<0.001 versus DHA treatment
