Figure 5
ARRDC4 interacts with MDA5 via arrestin-like N domain. (a and b) The t-MØ were infected with EV71, immunoprecipitation (IP) with anti-ARRDC4 (a) or anti-MDA5 (b) and control IgG antibodies. Immnoblot analysis of the interaction between ARRDC4 and MDA5; input, the whole-cell lysates without immunoprecipitated. (c and d) HEK293T cells were transfected with Flag- or Myc-tagged ARRDC4 and MDA5 plasmids, after 48 h, IP with anti-Flag antibodies, then detected protein interaction by immunoblot. (e) Schematic structure of ARRDC4 and the derivatives were shown, full-length ARRDC4 (ARRDC4-WT), arrestin-like N domain (ARRDC4-N), arrestin-like C domain (ARRDC4-C), PPXY motif (ARRDC4-PPXY). The IP and immunoblot analysis of the interaction between MDA5 and the indicated ARRDC4 constructs in HEK293T cells. Data are representative of three independent experiments with similar results
