Figure 6 | Cell Death & Disease

Figure 6

From: Autophagy blockade and lysosomal membrane permeabilization contribute to lead-induced nephrotoxicity in primary rat proximal tubular cells

Figure 6

Pb attenuated the proteolytic activity in lysosomes of rPT cells. (a) Cells were pre-incubated with 10 μg/ml DQ-BSA Green for 12 h, then refreshed the medium and treated with 0.5 μM Pb for 12 h, 50 μM CQ (negative control) for 3 h, or EBSS medium (positive control) for 2 h. Images were obtained by confocal microscopy. (b) Cells were treated with 0.5 μM Pb for 12 h, then harvested to assess the protein levels of CTSB and CTSD using the whole-cell lysates. Upper panel represent western blot image; lower panel represent quantitative analysis of protein levels (mean±S.E.M., n=4). **P<0.01

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