Figure 6
The Nrf2 signaling is required for effect of ESI in inducing autophagy. (a–c) H1299 and A549 cells transfected with anti-Nrf2 siRNA (50 nM) or control siRNA were treated with ESI, western blot assays were used to determine the expression levels of p62 and HO-1 (a) and the autophagy markers including Beclin1, ATG3 and LC3 (b), and the cell viability was measured by WST-1 assay (c). Note that ESI-induced protective autophagy and cell viability can be attenuated by knockdown of Nrf2. (d) Both H1299 and A549 cells were pretreated with 3-MA (2 mM) and then exposed to ESI (1.6 μM for H1299, 3.2 μM for A549) for another 24 h, the mRNA levels of p62 and HO-1 were determined by qRT-PCR. All data were representative of three independent experiments. Bars, S.E.M.; *P<0.05, **P<0.01, ***P<0.001; Nonsignificant is referred as n.s
