Figure 1
From: EGR-1/ASPP1 inter-regulatory loop promotes apoptosis by inhibiting cyto-protective autophagy
EGR-1 promotes ASPP1 expression at both mRNA and protein levels (a–c) Protein levels of ASPP1, EGR-1 and p53, a known EGR-1 target, were measured by western blotting (WB) at 0, 8, 12 and 24 h post-transfection with plasmid expressing EGR-1 or empty vector controls. β-actin was used as loading controls (a). Meanwhile, the mRNA levels of EGR-1 (b) and ASPP1 (c) were detected by real-time RT-PCR. (d–f) Protein levels of ASPP1, EGR-1 and p53 were measured by WB analysis after transfection with different doses of EGR-1 plasmids. β-actin was used as loading controls (d). Meanwhile, the mRNA levels of EGR-1 (e) and ASPP1 (f) were detected by real-time RT-PCR. (g and h) ASPP1 and EGR-1 were detected by WB in HCT116 cells both untreated and transfected with Si-EGR-1 or Si-Control. β-actin was used as loading controls (g). mRNA levels of EGR-1 and ASPP1 were measured by real-time RT-PCR after EGR-1 knockdown (h). *P<0.05
