Figure 2
From: EGR-1/ASPP1 inter-regulatory loop promotes apoptosis by inhibiting cyto-protective autophagy
ASPP1 is activated by the endogenous EGR-1 in response to various stimuli. (a–c) Expression levels of ASPP1 and EGR-1 were determined by WB analysis (a) and real-time RT-PCR (b and c) at different time points after Quercetin treatment in HCT116 cells. (d) Expression levels of ASPP1 and EGR-1 were determined by WB analysis in HCT116 cells after exposure to H2O2 (500 μM) for 4 h. (e and f) Cells were exposed to UV (40 J/mn) for the indicated time periods. Expression levels of ASPP1 and EGR-1 were determined by WB analysis in 293T (e) and H1299 (f) cells. β-actin was used as loading controls for all WB assays. *P<0.05, ***P<0.001
