Figure 6
From: EGR-1/ASPP1 inter-regulatory loop promotes apoptosis by inhibiting cyto-protective autophagy
EGR-1/ASPP1 inhibits cyto-protective autophagy. (a) Expression levels of ASPP1 and EGR-1 and autophagy protein marker LC3BI/II, were determined by WB analysis in HCT116 cells treated with Quercetin for the different time courses. β-Actin was used a loading control. (b) The expression of ASPP1, EGR-1 and LC3BI/II were determined by WB analysis in the presence or absence of Quercetin in HCT116/Shnon and HCT116/ShASPP1 stable lines. β-Actin was used as a loading control. (c) The expression of ASPP1 and LC3BI/II were determined by WB analysis in the presence or absence of Quercetin in HCT116 cells transfected with Si-Control or Si-EGR-1. β-Actin was used as a loading control. (d) GFP-LC3 puncta (⩾4puncta/cell) containing autophagy cells were counted in Si-ASPP1 or Si-EGR-1 transfected cells, in the presence or absence of Quercetin treatment. *, in comparison with DMSO-treated Si-controls. #, in comparison with Quercetin-treated Si-controls. (e) ASPP1 was re-expressed after SiRNA-mediated EGR-1 knockdown. The expression levels of ASPP1, EGR-1 and autophagy protein marker LC3BI/II, were determined by WB. β-Actin was used as a loading control
