Table 1 Primer sets used for quantitative real-time PCR
Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
IL6 | CCTTCCAAAGATGGCTGAAA | CAGGGGTGGTTATTGCATCT |
IL8 | ATGACTTCCAAGCTGGCCGTGGCT | TCTCAGCCCTCTTCAAAAACTTCTC |
Tnfα | CCCAGGGACCTCTCTCTAATC | ATGGGCTACAGGCTTGTCACT |
Snail | ACCACTATGCCGCGCTCTT | GGTCGTAGGGCTGCTGGAA |
Slug | TGTTGCAGTGAGGGCAAGAA | GACCCTGGTTGCTTCAAGGA |
CD44 | CCGCTATGTCCAGAAAGGA | CTGTCTGTGCTGTCGGTGAT |
CD133 | ACGCACGTAGGGAATGGAT | GGTTTGCACGATGCCACTTT |
c-myc | TCAAGAGGCGAACACACAAC | GGCCTTTTCATTGTTTTCCA |
Sox2 | AAGGGTTCTTGCTGGGTTTT | AGACCACGAAAACGGTCTTG |
Lgr5 | CTCTTCCTCAAACCGTCTGC | GATCGGAGGCTAAGCAACTG |
Oct4 | GTTGATCCTCGGACCTGGCTA | GGTTGCCTCTCACTCGGTTCT |
Nanog | GTCTTCTGCTGAGATGCCTCACA | CTTCTGCGTCACACCATTGCTAT |
TLR4 | CAGAGTTGCTTTCAATGGCATC | AGACTGTAATCAAGAACCTGGAGG |
TLR9 | TGGTGTTGAAGGACAGTTCTCTC | CACTCGGAGGTTTCCCAGC |
MyD88 | GACCCCTGGTGCAAGTACC | AGTAGCTTACAACGCATGACAG |
Trif3 | ACGCCACTCCAACTTTCTGT | TCAGGTGAGCTGAACAAGGA |
Nod1 | TCCAAAGCCAAACAGAAACTC | CAGCATCCAGATGAACGTG |
Rip2 | CCATCCCGTACCACAAGCTC | GCAGGATGCGGAATCTCAAT |
CK20 | CAGACACACGGTGAACTATGG | GATCAGCTTCCACTGTTAGACG |
CDX1 | TCGGACCAAGGACAAGTACC | TGTTGCTGCTGCTGTTTCTT |
GAPDH | GGTTTCTATAAATTGAGCCCGCA | ACCAAATCCGTTGACTCCGA |
18S rRNA | GGAGTATGGTTGCAAAGCTGA | ATCTGTCAATCCTGTCCGTGT |
